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comGC [2020-01-26 12:40:20]
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comGC [2020-01-26 12:40:20]

DNA uptake, major component of the pseudopilus
locus
BSU_24710
pI
6.32
mw
10.71 kDa
protein length
98 aa Sequence Blast
gene length
297 bp Sequence Blast
function
genetic competence
product
major pseudopilin
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.7|Genetic competence]
  • [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.3|Genetic competence]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

    • Gene

    Coordinates
    2,557,673 2,557,969

    Phenotypes of a mutant

  • knockout has complete loss of transformation competence [Pubmed|1968455]

    • The protein

    Protein family

  • comGC family (single member, according to UniProt)

    • [SW|Localization]

  • cell surface [Pubmed|9723928]
  • localizes to the cell membrane and the cytoplasm [Pubmed|21278288]
  • localizes to one single cell pole in the majority of the cells [Pubmed|21278288]

    • Expression and Regulation

    Operons

    genes
    [gene|41DFEF8A6BC7E34A6681C26D92F2B08DC120A957|comGA]-[gene|EA1C841FC77A1994A85E2E0C0A554482C3EC9323|comGB]-[gene|19D76B80386F825109C5FB4A34881D5D20F366DB|comGC]-[gene|D323AEF5AFB4FD2A074ABAB76A39BE26A4C6436F|comGD]-[gene|FF4DB7FDCEB440064E11E65CB0DBFB93FC785CF0|comGE]-[gene|DD266DC0D78B5C5E82EED7CC7F55D0CF8FB4BF7A|comGF]-[gene|89442BFDFBFF264D0C7F12DEA6F8130C55D1F3F8|comGG]-[gene|8F3751A3D0910C9BED7FBCE90AFF7FDACFDCBC0B|spoIIIL]
    description
    [Pubmed|26735940,2507524]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|2507524], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
  • [protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|SigF]: sigma factor, [Pubmed|26735940], in [regulon|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|SigF regulon]

    • regulatory mechanism

  • [protein|08CFA2C72931A75532D4289BC1D18A826DE9F9CA|ComK]: activation, [Pubmed|8196543], in [regulon|08CFA2C72931A75532D4289BC1D18A826DE9F9CA|ComK regulon]

    • regulation

  • expressed early during sporulation in the forespore ([protein|search|SigF]) [Pubmed|26735940]

    • additional information

  • the ''[SW|comGA]-[SW|comGB]-[SW|comGC]-[SW|comGD]-[SW|comGE]-[SW|comGF]-[SW|comGG]-[protein|search|spoIIIL]'' operon requires [SW|NusA] for expression [http://www.nature.com/articles/nmicrobiol20157 Reference]
    • view in new tab

    Biological materials

    Mutant

  • BKE24710 ([gene|19D76B80386F825109C5FB4A34881D5D20F366DB|comGC]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE24710 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTTCTCATTCATCAGCCTCT, downstream forward: _UP4_ATCATCACCGGCGGAGAAGT
  • BKK24710 ([gene|19D76B80386F825109C5FB4A34881D5D20F366DB|comGC]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK24710 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTTCTCATTCATCAGCCTCT, downstream forward: _UP4_ATCATCACCGGCGGAGAAGT
  • BP914 (([gene|41DFEF8A6BC7E34A6681C26D92F2B08DC120A957|comGA]-[gene|EA1C841FC77A1994A85E2E0C0A554482C3EC9323|comGB]-[gene|19D76B80386F825109C5FB4A34881D5D20F366DB|comGC]-[gene|D323AEF5AFB4FD2A074ABAB76A39BE26A4C6436F|comGD]-[gene|FF4DB7FDCEB440064E11E65CB0DBFB93FC785CF0|comGE]-[gene|DD266DC0D78B5C5E82EED7CC7F55D0CF8FB4BF7A|comGF]-[gene|89442BFDFBFF264D0C7F12DEA6F8130C55D1F3F8|comGG])::phleo), available in [SW|Fabian Commichau]'s lab

    • References

    Reviews

  • 15083159

    • Original publications

  • 11744713,11814663,9422590,16751195,9723928,1968455,7783624,21278288,12107147,2507524,8196543